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Supplementary information The transketolase active site, having a network of conserved His residues comes with a potent selection screen for miscoding His responding on the Trp codon UGG.
Transfer RNA recognition by aminoacyl tRNA synthetases.
Biochemistry 33, 1944–1950 (1994).
Maintaining this metabolic selection under conditions of permanent development in a turbidostat allowed us to validate the genetic stability from the Trp30 residue over 2500 generations.
Electrical systems, Trp substitution with the conserved site His481 was reproducibly accompanied by recovery of activity through point mutation, to Gly481, within 160 generations.
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